Plasmid_Backbone
Part:BBa_K4133000:Design
Designed by: Luis Alejandro Chaves Martinez Group: iGEM22_Costa_Rica (2022-10-02)
pLBov plasmid (Lactobacillus/E. coli shuttle vector)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4975
Illegal NheI site found at 2290
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4981 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4975
Illegal BglII site found at 2892
Illegal XhoI site found at 3959
Illegal XhoI site found at 4851 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4975
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4975
Plasmid lacks a suffix.
Illegal XbaI site found at 4990
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
Due to the cost issues related to vector synthesis, pLBov was synthesized in fragments and assembled using Single-Pot Scarless Golden Gate assembly with the following overhangs:
pLBov fragment | Prefix | Suffix | Location (nt) |
---|---|---|---|
1 | GGTCTC A CAGT | AACT T GAGACC | |
2 | GGTCTC T AACT | TCGT C GAGACC | |
3 | GGTCTC G TCGT | TAGG C GAGACC | |
4 | GGTCTC A TAGG | CAGT C GAGACC |
Source
This plasmid was obtained by de-novo synthesis.